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【文獻(xiàn)標(biāo)題】Effects of rearing systems on reproductive hormones secretion and their receptors gene expression in Xianju chickens under summer conditions

【作者】Z. Z. Yin，X. Y. Dong，H. Y. Cao，et.al

【作者單位】浙江大學(xué)動物科學(xué)學(xué)院（Animal Science College, Zhejiang University）

【文獻(xiàn)中引用產(chǎn)品】

放射免疫分析試劑盒

【關(guān)鍵詞】Xianju chicken, reproductive hormone, gene expression, rearing system, summer conditions

【DOI】dx.doi.org/10.3382/ps/pey194

【影響因子(IF)】3.5

【出版期刊】《Poultry Science》

【產(chǎn)品原文引用】

Blood sampling and analysis

At the end of wk 29, 12 h after feed withdrawal, 2 birds were randomly selected from each replicate and blood samples were collected from the axillary vein.Serum was isolated by centrifugation of blood at 3000×g for 10 min, and was stored in 1.5-mL Eppendorf tubes at −70?C until analyses. Serum FSH, P4, LH, and PRL levels were analyzed using RIA kits (Hengyuan Biological Technology Co., Ltd, Shanghai, China) by radio immunoassay, according to the recommendations of the manufacturer. For measurement of FSH concentration, the assay sensitivity, measuring range, intraand interassay CVs were 0.4 mIU/mL, 1.5 mIU/mL to 100mIU/mL, <10% and <15%, respectively. For measurement of P4 concentration, the assay sensitivity was 0.2 ng/mL, measuring range was 0.5 to 80 ng/mL, and the intra- and interassay CVs were <10%. For measurement of LH concentration, the assay sensitivity, measuring range, intra-and interassay CVs were 0.02, 0.5 to 20 ng/mL, <10% and <15%, respectively. For measurement of PRL concentration, the assay sensitivity was 0.5 ng/mL, measuring range was 5.0 to 100 ng/mL,and the intra- and interassay CVs were <10%. All the samples were measured in one single assay.

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